Have nuclear weapons ever been used?

Yes.

• In Hiroshima, Japan: deaths as of December 1945: 140,000 deaths
deaths calculated as of August 1996: 197,045 deaths

• In Nagasaki, Japan: deaths as of December, 1945: 74,000 deaths
 

In addition, 2,045 nuclear tests have resulted both in deaths and illness for people living in the test area and in serious environmental damage.

In the US alone, there are estimates of 49,000 deaths from radioactive fallout following nuclear tests at the Nevada Test Site during the 1950s and 60s.

http://rex.nci.nih.gov/massmedia/falloutQ%26A.html